CoronaMeltVAR is the new RT-PCR amplification kit for SARS-CoV-2 virus, designed for high sensitivity detection of viral RNA in samples derived from nasopharyngeal, oropharyngeal and SALIVA swabs allowing the simultaneous identification of the presence or absence of Omicron variants BA.1 and BA.2, distinguishing both from Delta. The assay is based on RT-PCR amplification with intercalating dye, followed by melting curve analysis to confirm the identity of the amplified product. CoronaMeltVAR is compatible with most RT-PCR instruments on the market, equipped with melting curve analysis capabilities and with magnetic bead or column RNA extraction systems. It can be used as a primary test or as a reflex test for a preliminary screening of positive samples to be sent for sequencing. The test includes three amplicons designed on SARS-CoV-2 targets located in the "ORF1ab" and "S" gene for indiscriminate detection of positivity to all known variants and the simultaneous presumptive identification of BA.1, BA.2, Alpha, Beta / Gamma and wild type / Delta, through the analysis of melting curves.
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