CoronaMeltVAR is a new Real Time PCR detection kit for SARS-CoV-2 virus, designed for high sensitivity detection of viral RNA in samples derived from nasopharyngeal and oropharyngeal swabs and simultanous identification of the presence of Alpha Beta and Gamma variants.
The assay is based on Real Time PCR amplification followed by melting curve analysis to confirm the identity of amplified product.
CoronaMeltVAR is compatible with most commercial Real Time PCR instruments having melting curve analysis capability and with Magnetic Bead or column RNA extraction methods.
The assay includes two amplicons designed on SARS-CoV-2 targets located in the ORF1ab gene for the indiscriminated detection of all known variants and two more amplicons designed to detect deletion ? 69/70 to identify the Alpha variant, or E484K mutation to identify Beta and Gamma. On a separate well an assay targets human GAPDH transcript, used as an endogenous control.
Brochure available here: